《植物生理学报》 2016, 52(2): 209-215
通信作者:沈应柏;E-mail: ybshen@bjfu.edu.cn
摘 要:
以拟南芥植株为材料, 观察了反式-2-己烯醛熏蒸处理后拟南芥初生根生长变化。采用非损伤微测技术, 从动态生长素流的角度研究了反式-2-己烯醛处理对拟南芥根尖生长素转运的影响, 并结合实时定量PCR技术检测了反式-2-己烯醛处理后生长素极性转运相关基因的表达。结果表明, 反式-2-己烯醛处理能够抑制拟南芥初生根生长, 且浓度越高抑制作用越强烈。反式-2-己烯醛显著抑制拟南芥根尖分生区及过渡区的生长素外排, 并显著下调了生长素极性转运基因PIN1、PIN2、PIN3及生长素感知基因TIR1的表达。说明反式-2-己烯醛抑制拟南芥根尖生长素的极性转运从而影响初生根生长。关键词:拟南芥; 反式-2-己烯醛; 生长素; 极性转运; 非损伤微测技术
收稿:2015-12-16 修定:2016-01-13
资助:国家自然科学基金(31270655)和国家“863”计划(2011-AA10020102)。 致谢 美国佛罗里达大学Eric Mclamore教授在生长素测定方面给予技术支持和指导, 中国农业科学院作物科学研究所傅永福研究员课题组在实时定量PCR方面给予技术支持。
Corresponding author: SHEN Ying-Bai; E-mail: ybshen@bjfu.edu.cn
Abstract:
In this study, primary root length of Arabidopsis thaliana seedlings was measured after E-2-hexenal fumigation. Using non-invasive micro-test technique, IAA flux rate was detected in different functional regions of the root apex with or without E-2-hexenal treatment. Furthermore, the expression levels of several polar auxin transport relevant genes were quantified by quantitative Real-time PCR. The result indicated that the primary root growth of Arabidopsis seedlings was strongly inhibited after E-2-hexenal exposure, and the inhibition was significant along with the increased concentrations of this aldehyde. E-2-hexenal significantly repressed the auxin efflux rate in the meristem and transition zone of seedling roots. E-2-hexenal also down-regulated the transcription expression level of auxin transport-related genes PIN1, PIN2, PIN3, and the IAA perception gene TIR1. Therefore, E-2-hexenal has the negative effect on polar auxin transport in roots, which finally inhibited the primary root elongation.Key words: Arabidopsis thaliana; E-2-hexenal; IAA; polar auxin transport; non-invasive micro-test technique
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